Detection of porcine endogenous retrovirus (PERV) using highly specific antisera against Gag and Env
نویسندگان
چکیده
منابع مشابه
Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR
Porcine endogenous retroviruses (PERVs) are members of family Retroviridae, genus Gamma retrovirus, and transmitted by both horizontally and vertically like other endogenous retroviruses (ERVs). PERV was initially described in the 1970s having inserted its gene in the host genome of different pig breeds, and three classes, PERV-A, PERV-B, and PERV-C are known. The therapeutic use of living cell...
متن کاملPorcine endogenous retrovirus (PERV) infection of HEK-293 cell line alters expression of human endogenous retrovirus (HERV-W) sequences.
The risk of infections of human recipients after xenotransplantations is now mainly represented by porcine endogenous retroviruses (PERVs) as these particles are part of the porcine genome. As in all vertebrates, human genome harbours its own numerous genetic sequences of retroviral origin; it is estimated that they comprise about 8 % of the human genome. Because some of them play an important ...
متن کاملCharacterisation of a human cell-adapted porcine endogenous retrovirus PERV-A/C.
BACKGROUND Porcine endogenous retroviruses (PERVs) pose a potential risk for xenotransplantation using pig cells, tissues or organs. A special threat comes from viruses generated by recombination between human-tropic PERV-A and ecotropic PERV-C. Serial passages of a recombinant PERV-A/C on human 293 cells resulted in increased infectious titers and a multimerization of transcription factor bind...
متن کاملDevelopment of sensitive methods for detection of porcine endogenous retrovirus-C (PERV-C) in the genome of pigs.
Porcine endogenous retroviruses (PERV) represent a risk for xenotransplantation using pig cells, tissues or organs. PERV-A and PERV-B are present in the genome of all pigs and both infect human cells in vitro. PERV-C infects only pig cells and it is integrated in the genome of most, but not all pigs. Recombinants between PERV-A and PERV-C were described that infect human cells and replicate at ...
متن کاملCan Antiretroviral Drugs Be Used to Treat Porcine Endogenous Retrovirus (PERV) Infection after Xenotransplantation?
Porcine endogenous retroviruses (PERVs) are integrated in the genome of all pigs; they are released as infectious particles, and under certain conditions they can infect human cells. Therefore, they represent a risk when pigs are used as sources of cells, tissues, or organs for xenotransplantation. Xenotransplantation is under development due to the increasing shortage of human transplants. Whe...
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ژورنال
عنوان ژورنال: Virology
سال: 2003
ISSN: 0042-6822
DOI: 10.1016/s0042-6822(03)00139-9